P-084 Oral antioxidant supplementation ameliorates the deleterious effects of plasticizers Bisphenol AF (BPAF) and Dibutyl Phthalate (DBP) on mouse sperm DNA integrity

Abstract Study question Do common chemicals in plastic, BPAF and DBP, negatively impact sperm parameters DNA integrity, can oral antioxidant supplementation mitigate their impact? Summary answer Short-term, low-dose exposure to or DBP did not testicular/epididymal histology but caudal epididymal parameters, particularly integrity was significantly compromised. What is known already Plasticizers are among the most commonly produced worldwide with 90% of people showing detectable levels urine. As endocrine disruptors (EDCs), they act as estrogen mimetics also induce oxidative stress form adducts. Numerous studies have linked EDCs impaired spermatogenesis, damage male infertility. Limited suggest antioxidants may alleviate reproductive toxicity EDCs. This study measured effects short-term exposures two environmental (BPAF & DBP) on post-testicular compartment epididymis investigate efficacy an supplement safeguard integrity. design, size, duration Adult CD1 mice were exposed low doses (50mg/kg) without co-administration (Fertilix®) for 14 days evaluated after euthanasia. Participants/materials, setting, methods All experiments performed at University Clermont Auvergne, Clermont-Ferrand, France. Mice supplemented BPAF, or/and Fertilix® drinking water. The results males against controls included: testicular histology, ROS status via 4-HNE caput protein extracts, general semen parameters: (count, motility, viability, acrosome integrity), nuclear/DNA [decondensation (Toluidine blue stain), fragmentation (TUNEL test), oxidation (8-OHdG residue level)]. Main role chance testis unaffected by conditions used. An situation evident EDC exposures, demonstrated increased level adducts extracts. Viability, total progressive spermatozoa remained unaltered animals compared controls. Exposure decreased count. Sperm assessed percentages decondensed oxidized nuclei EDC-exposed animals. increase any group Co-administration corrected defective (nuclear decondensation base oxidation) exception count which return control BPAF+ treated These data confirm that even susceptible induced ROS-generating EDCs, confirming its association Limitations, reasons caution translation these findings humans needs be confirmed, although nuclear human more alterations than mouse. Wider implications demonstrate susceptibility cells chemicals. They support use appropriate effective therapeutic choice individuals multiple stressors such Trial registration number applicable